Micro Baterial Morphology Lab

bacterial Morphology graphic symbol 1 Viewing Prep ard microscope slides of Common bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. lay out 2 Disinfecting Your subject area to Use Live Organisms Part 3 Viewing Live Organisms pixilated Mount Preparation in that location was several amoeba shaped carrels that change in size. There were five somewhat darker areas that were circular in shape. There were also three large, oddly shaped areas that had very distinct edges. Part 4 Direct Staining chute One There were 2 clusters that were fairly easy to recognize.All of the cells were cocci. Some of the cells were large while others were practically nonexistent. Slide Two There were different layers of cells. The cells were rectangular in shape and vary in size. A nucleus was visible in each cell. Slide Three This slide was a mixture of different shapes. Nothing was recogniza ble. Part 5 Indirect Staining Chains of twain cocci and bracillus cells were both visible and identifiable. The chains varied in length. The cheek and yeast calumniate was clearer. The same shapes were settlen as before just with sharper outlines.The cells were much easier to see with more detail. Questions A. What are the advantages of using bleach as a antimicrobial? The disadvantages? The advantages of using 70% alcohol? The disadvantages? B. List three reasons why you energy choose to stain a particular slide rather than take care it as a wonky mount. C. Define the following terms Chromophore sulfurous Dye Basic Dye D. What is the difference between direct and mediate staining? E. What is heat fixing? F. Why is it necessary to ensure that your specimens are completely air dried prior to heat fixing? G.Describe what you discovered in your plaque smear wet mount, direct stained slide, and indirectly stained slide. What were the quasi(prenominal)ities? What were the diffe rences? H. Describe what you observed in your cheek smear wet mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences? I. Describe what you observed in your yeast wet mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences? J. Were the cell types the same in all three specimen sets yeast, plaque, and cheek? How were they similar? How were they different?