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Lab depict 1Allison M. ShawBio 302L Sec 009Thursday February 12th, 2014IntroductionHL-60 cells were initially taken from a Caucasian female that had acute promyelocytic leukemia (Cirtain, Mittelstadt, Higgins, Pollson, 2002). These cells are good for analyze cellular processes and under favorable conditions, are immortal. HL-60 cells have a comparatively quick doubling time of no more than 48 hours, and do not experience apoptosis, which makes them immortal (Cirtain, et. al, 2002). The HL-60 cell disembowels immortality is implemental when it comes to finding possible treatments for patients with leukemia, which is of interest to researchers. This immortality attribute is also critical for studying mechanisms of monocyte and granulocyte differentiation (Collins, 1987). Another quality about the HL-60 cell flexure that is as important is its ability to key out into the lily-white blood cell line (Collins, 1987). Granulocytes and monocytes are the two most important types of differentiated cells in this experiment, although white blood cells also differentiate into macrophage-like cells and eosinophils (Collins, 1987). Compounds occurring naturally that induce HL-60 cell line differentiation include Vitamin D (Collins, 1987). Chemical inducers, such as DMSO and PMA in this experiment, can induce HL-60 cells to differentiate into cells along the myeloid path (Cirtain et al,. 2002). DMSO is canvas as a control for PMA treatment conditions and is used to make PMA solution. It causes HL-60 cells to differentiate mostly into granulocytes (Cirtain et al,. 2002). The control in this experiment is cells treated with DMSO, and is done to presentation DMSO has no effect on PMA (Cirtain et al,. 2002). Using kinase C, PMA activates cert... ...he line. Further studies could allow researchers to recognize valuable information possibly leading to cures for numerous cancer types. ReferencesCirtain, Mittelstadt, Higgins, Pollson. Chapter 3. place Point. cellpho ne and Molecular Biology Laboratory Manual. Great River Tech. Web. 8 Feb 2014. Cirtain, Mittelstadt, Higgins, Pollson. Chapter 4. occasion Point. cubicle and Molecular Biology Laboratory Manual. Great River Tech. Web. 8 Feb 2014. Collins, S. J. The HL-60 Promyelocytic Leukemia Cell Line Proliferation, Differentiation, and Cellular Oncogene vista. The Blood Journal 70 (1987) 1233-1244. Xie, Bei, Amale Laouar, and Eliezer Huberman. Fibronectin-mediated Cell Adhesion Is Required for Induction of 92-kDa Type IV Collagenase/Gelatinase (MMP-9) Gene Expression during Macrophage Differentiation. The Journal of Biological Chemistry (1998) 1576-1582.